Various Approaches of Molecular Process for Diagnosis of Viral Disease

The emergence and re-emergence of various viral diseases indicate the need for the development of effective and authentic new diagnostic methods. The diagnostic application of gel-based PCR, general PCR systems, phylogeny, molecular epidemiology, real-time PCR, amplification without thermo-cycling, multiplex PCR, nucleic acid extraction and pipetting robotics, automation and quality control, including internal controls plays significant role for laboratory diagnosis of viral disease. The development of various probes and microarrays, as well as ultra rapid PCR and sequencing methods is further improving the arsenal of nucleic acid based molecular diagnosis for viruses. Further trends of diagnostic development are also mentioned, in order to combat viral infections more effectively in the future.